The previous article introduced several reasons why cells do not adhere to the wall when using cell culture flasks and other containers. So what should be done if the effect of cell adherence culture is good? Let me give you a brief introduction.

If you want the effect of cell adherent culture to be good, you should do the following:

 1. Moderately digest cells;

 2. It is recommended to use surface-treated disposable plastic containers for cultivation;     

 3. Use appropriate extracellular matrix or attachment reagents;

 4. Resuscitate new cells, use 20% serum in the first week to help cells recover;

 5. Subculture inoculation must be spread evenly to avoid cell cluster growth;

 6. Try not to shake the cells within 24 hours of subculture, so as not to affect the adherence.