With the rapid development of various technologies, cell culture consumables are constantly updated and iterative, and baffle shaker is a relatively novel cell culture consumable. Standard triangle shaker, what are the differences between the two?
First of all, from the shape of the two are triangular design, the bottle cap is also divided into two kinds of sealed cap and breathable cap, the specification is roughly the same. The main difference between the two is the bottom of the bottle. Ordinary shakers have a flat bottom, while baffle shakers have grooves in the bottom, and the raised parts of these grooves form a baffle inside the bottle, hence the name.
The special design of baffle shaker has two functions. First, it can reduce the phenomenon of cell clump. When using the shaker, it can effectively reduce the adhesion caused by free DNA and cell debris and reduce the occurrence of cell clump growth. In addition, the baffle at the bottom can prevent the vortex phenomenon caused by the oscillation of the medium and make the medium more uniform, which also plays a role in reducing cell clumping. Second, it can increase dissolved oxygen. The baffle at the bottom of the bottle can increase the dissolved oxygen in the medium, effectively promote the cells to fully contact with the air, and help cells to grow better.
In general, the difference between baffle shakers and ordinary shakers is mainly the difference at the bottom of the bottle. The new bottle increases dissolved oxygen and is more suitable for cell lines with a high demand for oxygen.
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Post time: Dec-13-2022