Cell passage culture refers to the process of dividing the culture into small parts and re-inoculating it into another culture vessel (bottle) for further culture. High efficiency cell shaker is a common consumable for suspension cell culture, so how to use high efficiency cell shaker for cell passage?

By their nature, suspension cells are non-adherent, so no enzymes are needed to detach them from the surface of the high-efficiency shaker. In general laboratory, direct passage and centrifugal passage are commonly used to carry out the passage of suspended cells. When cells are observed to be 80 to 90 percent overgrown (the cell suspension turns yellow), the cells are ready for passage.

If the cells are growing well under a microscope, direct passage can be used. The medium in the high-efficiency shaking flask was proportionally divided into the new culture flask, and the fresh medium was added. The cell density was observed the next day to determine whether the fluid was needed.

If the cell condition is poor, the centrifugal passage method should be used. Firstly, the cell suspension is transferred to the centrifuge tube, centrifuged at 1000rpm for 5 min, then the supernatant is discarded, the cell precipitates are gently dispersed, and the cells are re-suspended with fresh medium. Finally, the appropriate amount of cell suspension is absorbed with a straw, put into a new culture bottle, and the appropriate amount of fresh medium is added. Continue to cultivate.

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