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How to choose ordinary centrifuge

Centrifuge is one of the basic equipment commonly used in laboratories, which is widely used in hospital laboratories. Centrifuge 10 is an indispensable tool for separating serum, precipitated tangible cells, PCR test and so on. Intelligent electric centrifuge has beautiful shape, large capacity, small size and complete functions. It has the advantages of stable performance, adjustable speed and automatic adjustment balance, low temperature rise, high efficiency and wide applicability. The intelligent electric centrifuge is suitable for qualitative analysis of serum, plasma and urea in medical products, blood stations, clinical trials and biochemical laboratories.

Choose ordinary centrifuges, according to the size of the workload, mainly from two aspects of speed and capacity. The following details the purchase of precision centrifuges should pay attention to the problems:

1. Speed
Centrifuges are divided into low-speed centrifuges <10000rpm/min, high-speed centrifuges 10000rpm/min ~ 30000rpm/min, and ultra-high-speed centrifuges >30000rpm/min according to the maximum speed. Each centrifuge has a rated maximum speed, and the maximum speed refers to the speed under no-load conditions. However, the maximum speed varies according to the type of rotor and the size of the sample mass. For example, the rated speed of a centrifuge is 16000rpm/min, indicating that the rotor rotates 16,000 times per minute when the load is not loaded, and the speed will certainly be less than 16000rpm/min after adding the sample. Different rotor, the maximum speed is also different; An imported centrifuge can be selected with a number of rotors, and a few manufacturers of domestic centrifuges have successfully developed such technology, such as TG16 desktop high-speed centrifuges, TGL16, TGL20 desktop high-speed refrigerated centrifuges, and many other models can be loaded with 16 kinds of rotors, which can be used in one machine. The horizontal rotor can reach 15000rpm/min, but the Angle rotor can reach about 14000rpm/min, the specific difference to consult the product sales personnel and the relevant technical personnel of the production plant in detail, so the choice of speed should be careful, the maximum speed of the selected centrifuge should be higher than the target speed. For example, if the target speed is 16000rpm/mIn, the maximum speed of the selected centrifuge must be higher than 16000rpm/min. Generally, the separation effect mainly depends on the speed, but the centrifugal force, so sometimes the speed does not meet the requirements, as long as the centrifugal force can reach the standard, the experiment can achieve the effect you need.

Centrifugal force calculation formula: RCF=11.2×R× (r/min/1000) 2    R represents the centrifugal radius, r/min represents the speed

2. Temperature
Some samples such as proteins, cells, etc. will be destroyed in high temperature environments, which requires the choice of frozen centrifuges, which have a rated temperature range. Centrifuge at high speed when the heat generated and centrifuge refrigeration system balance at a certain temperature, generally frozen centrifuge samples need to be maintained at 3 ° C ~ 8 ° C, the specific amount can be achieved and the rotor, such as a centrifuge rated temperature range of -10 ° C ~ 60 ° C, install the horizontal rotor can reach about 3 ° C when rotating, If it is an angular rotor, it may only reach about 7 ° C. This point should also consult the product sales personnel and the relevant technical personnel of the production plant in detail.

Capacity

3. Capacity
How many sample tubes should be centrifuged at a time? How much capacity does each sample tube need?
These factors determine the total capacity of a centrifuge, simply speaking, the total capacity of the centrifuge = the capacity of each centrifugal tube × the number of centrifugal tubes, the total capacity and the size of the workload is matched.

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Post time: Jun-19-2023