In suspension cell culture, cell shake flask is a kind of cell culture consumable. The growth of suspended cells did not depend on the surface of the supporting material and they grew in suspension state in the culture medium. How do we determine the amount of liquid to be added in real culture?

Common specifications of cell shaker include 125ml, 250ml, 500ml and 1000ml to meet the needs of different sizes of cell culture. For example, 125ml and 250ml bottles with small capacity are mainly used for small-scale experiments, while 500ml and 1000ml specifications are used for medium-scale cell culture experiments. When using this kind of consumables, the vibration of the shaker should be used to reduce the cell agglomeration rate and maintain the good growth state of the cells. Cell culture needs to be carried out in a sterile environment. Therefore, the triangle culture flask will be specially sterilized before being put into use to achieve the effect of no DNase, no RNA enzyme, and no animal-derived components, providing a good environment for cell growth.

According to the different specifications of the bottle, the recommended filling volume of the four specifications of the bottle from low to high is 30ml, 60ml, 125ml, 500ml. Generally, the volume of solution in the culture of cells is controlled at about 20%-30% of the total volume of the shaking bottle, and there is a clear scale line on the bottle body to facilitate visual observation of the capacity of the solution.

The above is the recommended amount of liquid added to different specifications of cell shaker, which is not fixed. The capacity should be considered comprehensively according to the characteristics of cell growth and inoculation density, so as to avoid the influence of cell growth due to the excessive amount of liquid added.