When we use cell culture bottles to culture cells, once contamination is found, it will affect the later growth, and it is difficult to eradicate the contamination. It is suggested to discard the contamination after elimination, so as not to affect the final experimental effect. So to avoid cell contamination, prevention is key:

1. Operator: Wear lab clothes when entering the training room, keep your hands clean, wear gloves, do not touch irrelevant items, avoid wearing watches, rings and other jewelry, sick people need to wear a mask.

2. Operation specifications: Establish daily disinfection specifications for aseptic room and CO2 incubator, regularly check whether the equipment is contaminated, and strictly implement various aseptic operations during the culture process.

3. to prevent cross infection: in a variety of cell culture operations, the use of cell culture bottles and AIDS should be strictly distinguished.

4. Cell culture bottles: Cell culture bottles are an essential tool in cell culture and a cause of contamination. Although the glass material consumables can be reused, they are easy to be sterilized incompletely in the process of self-sterilization. In order to avoid pollution, it is recommended to use disposable pre-sterilized cell culture consumables as far as possible.

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