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Characteristics of cell shaker and matters needing attention in use

Cell culture is also called cell cloning technology, is an important technical means of biological research. Cell shaker is a kind of special consumable material used in the process of cell culture. It is the premise of cell culture to understand the characteristics of cell shaker and use precautions. Cell shakers are generally made of BPA-free PC material or PETG material through injection, drawing and blowing process, with the following characteristics:

1. 2.8L and 5L cell shaker caps have a larger breathable film area than similar products, which is suitable for high density cell culture. The working volume can be filled to 60-80% of the total volume, and the shaker caps with the same volume have a higher cell output.

2.The arc design of the 2.8L bottle neck is too natural. The size of the neck not only ensures the effective gas exchange space, but also facilitates the operation of single hand holding.
3. 5L bottle with ergonomic design of the handle, easy to shake the bottle and liquid transfer.
4.the standard product of the bottle is equipped with 0.2μm sterilizing breathable. In addition, the liquid transfer cap is optional to facilitate the aseptic transfer of liquid, and the bottle cap can also be customized to match the liquid transfer according to the process requirements of customers.
5.the bottle cap breathable film hydrophobic design, and the liquid contact does not affect the sealing and breathable effect of the breathable film.

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When using the shaker, it is necessary to match the shaker for cell culture, and the solvent should not exceed 30%-40% of the shaker. During the culture process, attention should be paid to the control of the speed. The general initial speed is 75-125RPM, which can be adjusted according to the specific situation. Air jacket type shaker needs to pay attention to temperature control, water jacket type shaker pay attention to water level.

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Post time: Dec-08-2022