Cell culture bottle  is a kind of cell culture consumable which is widely used in cell culture experiment, and it is mostly used in adherent cell culture. One of the important factors to examine this kind of consumable is the adhesion property, which is related to whether the cells can stick to the surface of the bottle well.

Cell culture bottles  are made of polystyrene, a transparent polymer material. According to the different needs of cell growth, they are equipped with ordinary sealed caps and hydrophobic filter caps, which can effectively prevent pollution while exchanging gas. The wall adhesion performance of bottle is mainly affected by two factors:

1. Surface treatment process: When used for adherent cell culture, the cell culture bottle will undergo special surface treatment before being put into use, and hydrophilic groups will be introduced on the surface of the bottle to ensure that the cells can stick to the surface for growth. If the bottle wall adhesion performance is poor, there may be problems in the control of details in the surface treatment, and strict treatment process can ensure the product’s wall adhesion effect.
2. the material of the product: on the other hand, the wall performance is also related to the material selected by the product. The material of this consumable should meet the requirements of USPVI, which is a relatively strict test of plastic material in the medical field and pipeline products in the biopharmaceutical field. The consumable purity meeting this condition is relatively high, which can fundamentally improve the adhesion performance of the product.

These are the two main factors that affect the adhesion performance of cell culture bottle. In addition, if the adhesion performance of cell is poor, whether the cell itself is in a good state should be considered. If the cell is in a poor state, its adhesion effect will also be affected.

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